![]() ![]() Lachiewicz AM, Hauck CG, Weber DJ, Cairns BA, van Duin D. PMID: 33737924Ĭhurch D, Elsayed S, Reid O, Winston B, Lindsay R. Severe Altered Immune Status After Burn Injury Is Associated With Bacterial Infection and Septic Shock. Moins-Teisserenc H, Cordeiro DJ, Audigier V, Ressaire Q, Benyamina M, Lambert J, et al. Porcine models of cutaneous wound healing. Jeschke MG, van Baar ME, Choudhry MA, Chung KK, Gibran NS, Logsetty S. Accordingly, this would allow for the design and evaluation of more effective burn infection therapies leading to better strategies for patient care. This protocol aims to provide investigators with a step-by-step guide to execute a technically challenging porcine wound infection model in a reproducible manner. Finally, advice on animal housing considerations, efficient bacterial plating procedures, and overcoming common technical challenges is provided. Systematic evaluation of wound healing and bacterial colonization of the wound bed is also described. Enumerated are steps to achieve standardized wound creation, bacterial inoculation, and dressing techniques. Wound healing kinetics and bacterial clearance were measured over a period of 27 d in this model. #INFECTED BURN FULL#We present a detailed protocol for a porcine model of infected burn wounds based on our experience in creating and evaluating full thickness burn wounds infected with Staphylococcus aureus on six pigs. However, utilizing this large animal model for wound infection studies can be technically challenging and create issues with data reproducibility. #INFECTED BURN SKIN#Currently, pigs are used as the gold standard of pre-clinical models to study infected skin wounds due to the similarity between porcine and human skin in terms of structure and immunological response. Bacterial infections often complicate burn injuries, which presents unique challenges for wound management and improved patient outcomes. Severe burns are traumatic and physically debilitating injuries with a high rate of mortality. The Biological Research Unit, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USAĭepartment of Plastic Surgery, Dermatology & Plastic Surgery Institute, Cleveland Clinic, Cleveland, Ohio, USAīurn wounds, infection, porcine model, protocol, wound healing Abstract Department of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USAĭepartment of Biology, John Carroll University, University Heights, Ohio, USA ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |